Optimization of Culture Conditions for the Production of Extracellular Cellulase from Enterococcus pseudoavium

Authors

  • M. Sakthivel, N. Karthikeyan, J. Meenakshi and P. Palani*

Abstract

Bacteria that inhabit decaying vegetables were isolated and screened for the production of the enzyme, amylase. One species identified as Enterococcus pseudoavium exhibited relatively higher amylase activity of the bacterial species tested. The bacterium produced the highest extracellular amylase at 72 h in a medium containing starch (1% w/v), galactose (0.5% w/v), and peptone (0.5% w/v), at pH 7.0. The extracellular enzyme was partially purified and its starch hydrolyzing potential was evaluated. The organism when grown with paper pulp deinked the pulp completely just after four days of growth. The bacterial cells immobilized in sodium alginate beads when cultured with paper pulp could decolorize it within 4 days. The extracellular amylase produced by Enterococcus pseudoavium effectively deinked and decolorized paper pulp within 4 days after incubation. The enzyme efficiently removed the starch present in fabric and thus it could be very well used as an ingredient in commercial detergent.

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Author Biography

M. Sakthivel, N. Karthikeyan, J. Meenakshi and P. Palani*

Centre for Advanced Studies in Botany, University of Madras, Maraimalai Campus, Guindy, Chennai-600 025, India

Published

23-12-2010

How to Cite

Meenakshi and P. Palani*, M. S. N. K. J. “Optimization of Culture Conditions for the Production of Extracellular Cellulase from Enterococcus Pseudoavium”. Journal of Experimental Sciences, vol. 1, no. 11, Dec. 2010, https://updatepublishing.com/journal/index.php/jes/article/view/1768.

Issue

Section

Life Sciences