In vitro Regeneration and Phytochemical Screening of Tylophora indica, an Endangered Medicinal Herb

Abstract

ABSTRACT: In vitro regeneration of Tylophora indica from mature leaf explants placed on MS with low concentration (1mM) of Kin and BA alone and in combination with Kin and BA induced multiple shoots and roots. Leaf explants on MS with BA   and 2, 4-D or NAA along with Ads had poor organogenic responses. The responses of leaf explants in MS + Kin (1mM) implicate the feasibility for multiplication of this species. Comparative phytochemical analysis revealed the presence of alkaloids, steroids and carbohydrates both in adult leaf and in vitro derived callus. It evidences the potential utility of tissue cultures for production of bioactive compounds. It may be concluded that further standardization of hormonal combinations could   helpful for large scale propagation and extraction of drugs for pharmaceutical application.