In vivo and In vitro Estimation of Colchicine in Gloriosa superba L. by High Pressure Liquid Chromatography

Authors

  • Renu Sarin*, Anirudha Rishi and Ajit Kumar

Abstract

The presence of Colchicine in Gloriosa superba was confirmed by Thin Layer Chromatography (TLC) and High Pressure Liquid Chromatography (HPLC) in which a spot coinciding with the authentic sample of Cochicine in Rf value (Colchicine 0.70) appeared. The maximum amount of Colchicine was found in flower (20.7 mg/gdw) and minimum amount was found in stem (7.4 mg/gdw).The In vitro studies showed the maximum amount of Colchicine in 6 week old cultures (22.6 mg/gdw)) and minimum amount in 2 weeks old cultures (15.3 mg/gdw).

Key words: Colchicine, Gloriosa superba, HPLC

Laboratory of Bioactive Natural Products, Department of Botany, University of Rajasthan, Jaipur -302004

* Corresponding Author, Email: [email protected]; Tel: +91-141-2701875, +91-9828070584

Please Cite This Article As:

Renu Sarin, Anirudha Rishi and Ajit Kumar. 2010. In vivo and In vitro Estimation of Colchicine in Gloriosa superba L. by High Pressure Liquid Chromatography. J. Exp. Sci. 1(4):1-2.

 

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Author Biography

Renu Sarin*, Anirudha Rishi and Ajit Kumar

Laboratory of Bioactive Natural Products, Department of Botany, University of Rajasthan, Jaipur -302004 * Corresponding Author, Email: [email protected]; Tel: +91-141-2701875, +91-9828070584

Published

07-09-2010

How to Cite

Ajit Kumar, R. S. A. R. and. “In Vivo and In Vitro Estimation of Colchicine in Gloriosa Superba L. By High Pressure Liquid Chromatography”. Journal of Experimental Sciences, vol. 1, no. 4, Sept. 2010, https://updatepublishing.com/journal/index.php/jes/article/view/1709.

Issue

Section

Life Sciences