Isolation and screening of proteolytic bacteria from freshwater fish Cyprinus carpio

Authors

  • Balaji N Department of Microbiology, The Madura College (Autonomous), Madurai – 625 011, Tamilnadu, India.
  • Rajasekaran K M Department of Microbiology, The Madura College (Autonomous), Madurai – 625 011, Tamilnadu, India.
  • Kanipandian N Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli-620 024, Tamilnadu, India.
  • Vignesh V Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli-620 024, Tamilnadu, India
  • Thirumurugan R Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli-620 024, Tamilnadu, India

Keywords:

Protease, Bacillus sp., Cyprinus carpio, pH and temperature

Abstract

The present study was undertaken to isolate and characterize the protease producing potent bacteria from gastro intestinal tract of the freshwater fish Cyprinus carpio. The protease producer was characterized as Bacillus sp. morphologically and biochemically. The optimization studies for growth and proteases production of the isolate Bacillus sp. was carried out. The optimum temperature for the isolated Bacillus sp. was 350C but the optimum temperature for the protease production was 450C, the optimum pH was 7.0 in which the isolate produced 18 mMol/ml and showed a remarkable number of Colony Forming Unit. Hence, it was focused to study the intestinal Bacillus sp. in freshwater fish and its protease enzyme. 

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Author Biographies

Balaji N, Department of Microbiology, The Madura College (Autonomous), Madurai – 625 011, Tamilnadu, India.

Department of Microbiology, The Madura College (Autonomous), Madurai – 625 011, Tamilnadu, India.

Rajasekaran K M, Department of Microbiology, The Madura College (Autonomous), Madurai – 625 011, Tamilnadu, India.

Department of Microbiology, The Madura College (Autonomous), Madurai – 625 011, Tamilnadu, India.

Kanipandian N, Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli-620 024, Tamilnadu, India.

Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli-620 024, Tamilnadu, India. 

Vignesh V, Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli-620 024, Tamilnadu, India

Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli-620 024, Tamilnadu, India

Thirumurugan R, Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli-620 024, Tamilnadu, India

Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli-620 024, Tamilnadu, India

Published

15-06-2012

How to Cite

N, B., R. K. M, K. N, V. V, and T. R. “Isolation and Screening of Proteolytic Bacteria from Freshwater Fish Cyprinus Carpio”. International Multidisciplinary Research Journal, vol. 2, no. 6, June 2012, https://updatepublishing.com/journal/index.php/imrj/article/view/1619.

Issue

Vol. 2 No. 6 (2012)

Section

Research Articles