In vitro evaluation of bioagents for the management of basal stem rot disease of coconut
Abstract
Basal stem rot caused by Ganoderma spp. is an important disease affecting coconut. Being a soil-borne pathogen, it can persist in coconut ecosystem for longer periods. Therefore, an eco-friendly remedy for the management of Ganoderma is essential. Following soil sample collection and isolation, the current study evaluated the efficacy of fluorescent Pseudomonas and Trichoderma spp. against basal stem rot pathogen Ganoderma gibbosum in vitro using dual culture assay. Among the 15 isolates of fluorescent Pseudomonas evaluated, the inhibition varied between 41% and 80 %. P. aeruginosa (isolate P13) exhibited maximum inhibition of 80.36%. All the isolates of Trichoderma showed greater antagonistic capacity with percentage inhibitions ranging from 71% to 87 %. T. harzianum isolate T20 exhibited a maximum inhibition of 87.92%.The present study identified several efficient antagonists of Ganoderma spp that could be further utilized for the management of the pathogen in field conditions.
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Ariffin, D. and Idris, S. 1991. A selective medium for the isolation of Ganoderma from disease tissues. pp 517–519.In: Proceedings of the 1991 International palm oil conference, progress, Prospects and challenges towards the 21st Century. Palm Oil Research Institute of Malaysia, Selangor, Malaysia.
Bhaskaran, R., Ramadoss, N. and Suriachandraselvan, M. 1988. Effect of plant on the growth of Ganoderma spp. associated with Thanjavur wilt disease of coconut. p.24. In: Proceedings of National seminar on management of crop diseases with plant products / Biological agents, Madurai, Tamil Nadu.
Bhaskaran, R., Ramanathan, T. 1982 Management of Thanjavur wilt of coconut. pp.521-526. In: PLACROSYM V. CPCRI, Kasaragod.
CDB, 2021. Statistics for coconut. Horticulture Division, Department of Agriculture and Cooperation, Ministry of Agriculture, Govt. of India, Coconut Development Board. https://coconutboard.gov.in/Statistics.aspx. Accessed 18 Dec 2023.
Chandra, H., Kumari, P., Bisht, R., Prasad, R. and Yadav, S., 2020. Plant growth promoting Pseudomonas aeruginosa from Valeriana wallichii displays antagonistic potential against three phytopathogenic fungi. Molecular Biology Reports 47(8):6015-6026.
Deshwal, V.K., Vig, K., Amisha, Dwivedi, M., Yadav, P., Bhattacharya, D. and Verma, M. 2011. Synergistic effects of the inoculation with plant growth-promoting Rhizobium and Pseudomonas on the performance of Mucuna. Annals of Forestry 19(1): 13-20.
Dowling, D.N. and O'Gara, F. 1994. Metabolites of Pseudomonas involved in the biocontrol of plant disease. Trends in Biotechnology 12(4):133-141.
Elad, Y. and Chet, I. 1983. Improved selective media for isolation of Trichoderma spp. Phytoparasitica11(1):55-58.
George, P., Gupta, A., Gopal, M., Chandra Mohanan, R., Thomas, L. and Thomas, G.V. 2012. In vitro antagonism of rhizospheric fluorescent pseudomonads of coconut against Ganodermaapplanatum and Thielaviopsis paradoxa, fungal pathogens of coconut. Journal of Plantation Crops 40(2):75-81.
Haas, D. and Défago, G. 2005. Biological control of soil-borne pathogens by fluorescent pseudomonads. Nature reviews microbiology 3(4): 307-319.
He, J., Baldini, R.L., Déziel, E., Saucier, M., Zhang, Q., Liberati, N.T., Lee, D., Urbach, J., Goodman, H.M. and Rahme, L.G. 2004. The broad host range pathogen Pseudomonas aeruginosa strain PA14 carries two pathogenicity islands harboring plant and animal virulence genes. Proceedings of the National Academy of Sciences 101(8):2530-2535.
Heuer, H., Krsek, M., Baker, P., Smalla, K. and Wellington, E.M. 1997. Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients. Applied and Environmental Microbiology 63:3233–3241.
Hubballi, M., Maheswarappa, H.P., Siddappa, R. and Chandrashekara, G.S. 2019. Biological control agents for the management of basal stem rot disease in coconut. Journal of Mycology and Plant Pathology 49(1):89-97.
Karthikeyan, G., Karpagavalli, S., Rabindran, R. and Natarajan, C. 2005. Biological control of basal stem rot disease in coconut. Planter 81(957):777-784.
King, E.O., Ward, M.K. and Raney, D.E. 1954. Two simple methods for the determination of pyocyanin and fluorescin. Journal of Laboratory and Clinical Medicine 44:301-304.
Kotasthane, A.S., Agrawal, T., Zaidi, N.W. and Singh, U.S. 2017. Identification of siderophore producing and cynogenic fluorescent Pseudomonas and a simple confrontation assay to identify potential bio-control agent for collar rot of chickpea. 3 Biotech 7(2):1-8.
Kubicek, C.P., Mach, R.L., Peterbauer, C.K. and Lorito, M. 2001. Trichoderma: from genes to biocontrol. Journal of Plant Pathology 83(2):11-23.
Kullnig, C., Mach, R.L., Lorito, M. and Kubicek, C.P. 2000. Enzyme diffusion from Trichoderma atroviride (= T. harzianum P1) to Rhizoctonia solani is a prerequisite for triggering of Trichoderma ech42 gene expression before mycoparasitic contact. Applied and Environmental Microbiology 66(5):2232-2234.
Mukherjee, P.K., Wiest, A., Ruiz, N., Keightley, A., Moran-Diez, M.E., McCluskey, K., Pouchus, Y.F. and Kenerley, C.M., 2011. Two classes of new peptaibols are synthesized by a single non-ribosomal peptide synthetase of Trichoderma virens. Journal of Biological Chemistry 286(6): 4544-4554.
Naher, L., Tan, S.G., Yusuf, U.K., Ho, C.L. and Abdullah, F. 2012. Biocontrol agent Trichoderma harzianum strain FA 1132 as an enhancer of oil palm growth. Pertanika Journal of tropical agricultural science 35(1): 173-182.
Neeraja, B., Snehalatharani, A., Maheswarappa, H.P., Ramanandam, G., Rao, N.C. and Padma, E. 2018. Management of Basal Stem Rot (Ganoderma Wilt) in Coconut with effective bioagents under field condition. International Journal of current microbiology and applied sciences 7(9): 1051-1060.
Rajappan, K., Surulirajan, M., Natarajan, C. and Arulraj, S. 2010. Effect of Pseudomonas fluorescens and Trichoderma viride in the management of basal stem rot disease in coconut.pp 264-268. In: Improving productivity and profitability in coconut farming. (Eds.) Thomas, G.V. Krishnakumar, V. and Jerard, B.A. Central Plantation Crops Research Institute, Kasaragod.
Ramamoorthy, V., Raguchander, T. and Samiyappan, R. 2002. Enhancing resistance of tomato and hot pepper to Pythium diseases by seed treatment with fluorescent pseudomonads. European journal of plant pathology 108:429-441.
Ranjana, C. and Sarma, T.C. 2017. In vitro evaluation of bio-control agents and fungicides against Ganoderma lucidum causing basal stem rot of arecanut. Indian Phytopathology 70(3): 336-340.
RStudio Team. 2020. RStudio: Integrated development environment for R. RStudio, PBC: Boston, MA, USA. http://www.rstudio.com/.
Saravanakumar, D., Lavanya, N., Muthumeena, B., Raguchander, T., Suresh, S. and Samiyappan, R. 2008. Pseudomonas fluorescens enhances resistance and natural enemy population in rice plants against leaffolder pest. Journal of Applied Entomology 132(6):469-479.
Srinivasalu, B., Aruna, K., Sabitha, D. and Rao, D.V.R. 2001. Occurrence and Bio-control of Ganoderma wilt disease of coconut in Coastal Agro-Ecosystem of Andhra Pradesh. Journal of the Indian Society of Coastal Agricultural Research 19(1 and 2):191-195.
Srinivasalu, B., Aruna, K., Rao, D.V. and Hameed Khan H. 2003. Epidemiologyof basal stem rot (Ganoderma wilt) disease of coconut inAndhra Pradesh. Indian Journal of Plant Protection 31(1):48-50.
Srinivasulu, B., Sabitha Doraiswamy, S.D., Aruna, K., Rao, D.V.R. and Rabindran, R. 2002. Efficacy of bio-control agent, chemicals and botanicals on Ganoderma sp., the coconut basal stem rot pathogen.Journal of Plantation Crops 30(3): 57- 59.
Subramanian, P., Thamban, C., Hegde, V., Hebbar, K.B., Bhat, R., Krishnakumar, V., Niral, V. and Josephrajkumar, A., 2018. Coconut, Technical bulletin no. 133. ICAR-CPCRI, Kasaragod.
Vinale, F., Marra, R., Scala, F., Lorito, M., Ghisalberti, E.L. and Sivasithamparam, K. 2005. Secondary metabolites produced by two commercial strains of Trichoderma harzianum. Journal of Plant Pathology 87(4):267-306.
Vincent, J.M. 1947. Distortion of fungal hyphae in the presence of certain inhibitors. Nature 159(4051):850-850.
White, T.J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics.pp.315-322. In: PCR Protocols: A Guide to Methods and Applications. (Eds.)Innis, M.A.Gelfand, D.H. Sninsky, J.J. and White T.J. Academic Press, New York.
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