TY - JOUR AU - Neelam Lad1 and S. P. Pawar2, Kirti Pawar1*, PY - 2010/12/04 Y2 - 2024/03/29 TI - Simultaneous Spectrophotometric Estimation of 11β,17α-dihydroxy -4-pregnene-3,20-dione- 21-succinate and its 1(2)-Dehydrogenated Product During Bioconversion by Comamonas acidovorans MTCC 3364 JF - Recent Research in Science and Technology JA - RRST VL - 3 IS - 1 SE - Microbiology DO - UR - https://updatepublishing.com/journal/index.php/rrst/article/view/561 SP - AB - <p class="MsoNormal" style="line-height: normal; text-indent: 0in; margin: 0in 0in 0pt; tab-stops: center 2.75in right 5.5in left 6.25in;"><span style="font-family: ";Arial Narrow";,";sans-serif";; color: black; font-size: 10pt;">A spectrophotometric method for simultaneously estimating 11β,17α-dihydroxy-4-pregnene-3,20-dione 21-succinate and its 1(2)-dehydrogenated product has been developed using sulphuric acid chromogens. The method was used to follow the progress of bioconversion during C-1(2)-dehydrogenation by <em>Comamonas acidovorans</em> MTCC 3364. </span></p><p class="MsoNormal" style="text-indent: 0in; margin: 6pt 0in 0pt; tab-stops: center 2.75in right 5.5in left 6.25in;"><strong style="mso-bidi-font-weight: normal;"><span style="line-height: 110%; font-family: ";Arial Narrow";,";sans-serif";; color: black; font-size: 10pt;">Keywords</span></strong><span style="line-height: 110%; font-family: ";Arial Narrow";,";sans-serif";; color: black; font-size: 10pt;">: Steroid 1(2)-dehydrogenation; Simultaneous estimation; Steroid bioconversion; Hydrocortisone; Prednisolone estimation </span></p> ER -