TY - JOUR AU - D. Selvakumar1*, J. H. Jagannath1, Mallesha1,2, R. Shylaja1,2, PY - 2010/09/05 Y2 - 2024/03/29 TI - Isolation and Identification of Lactic Acid Bacteria from Raw and Fermented Products and their Antibacterial Activity JF - Recent Research in Science and Technology JA - RRST VL - 2 IS - 6 SE - Microbiology DO - UR - https://updatepublishing.com/journal/index.php/rrst/article/view/464 SP - AB - <p class="MsoNormal" style="margin: 0in 0in 0pt; text-indent: 0in; line-height: normal; text-align: justify; tab-stops: center 2.75in right 5.5in left 6.25in;"><span style="font-size: 10pt; color: black; font-family: ";Arial Narrow";,";sans-serif";;">Lactic acid bacteria (LAB) were isolated from raw and fermented products like milk, curd, idli batter and pickle. Out of 44 isolates, 16 species were identified.<span style="mso-spacerun: yes;">  </span>Among them <em style="mso-bidi-font-style: normal;">Enterococcus faecalis</em> was the dominant species (37.5%).<span style="mso-spacerun: yes;">  </span><em style="mso-bidi-font-style: normal;">Streptococcus pyogenes</em>, <em style="mso-bidi-font-style: normal;">Lactobacillus casei</em> and <em style="mso-bidi-font-style: normal;">Streptococcus viridans</em> accounted for 18.75%, 18.75% and 6.25% respectively.<span style="mso-spacerun: yes;">  </span>The remaining 18.75% isolates of <em style="mso-bidi-font-style: normal;">Lactobacilli</em> were found to be <em style="mso-bidi-font-style: normal;">Leuconostoc</em> sp. Bacteriocin like inhibitory substance (BLIS) from the four isolates except <em style="mso-bidi-font-style: normal;">Leuconostoc</em> sp. were tested against selected pathogens of both gram positive and gram negative group.<span style="mso-spacerun: yes;">  </span>BLIS of <em style="mso-bidi-font-style: normal;">E. faecalis</em> in combination with 0.1% Ethylene diamine tetra acetic acid disodium salt (EDTA) showed the best antibacterial activity among all the isolates tested.</span></p> ER -