In Vitro Egg Hatchability Inhibition Effect of Albizia gummifera, Phytolacca dodecandra, and Vernonia amygdalina against Natural Infection of Ovine GIT Nematodes

  • Bizuneh Tsehayneh Bahir Dar University, College of Agriculture and Environmental Sciences, Department of Veterinary Science, Bahir Dar, Ethiopia
  • Achenef Melaku University of Gondar, School of Veterinary Medicine and animal science, Department of Veterinary Medicine, Gondar, Ethiopia


An in-vitro experiment was carried out to evaluate the egg hatching inhibition effect of three herbal plants, namely;Albizia gummifera, Phytolacca dodecandra, and Vernonia amygdalina. The leaves of these plants were collected; air dried and powdered with pestle and mortar, and then hydro-alcoholic extraction was performed and in measuring the percentage yield, P. dodecandra givesbetter yield (15.34%). Crude extract of these plants were evaluated for egg hatchability assay at different concentrations (3mg/ml, 5mg/ml and 10mg/ml) for each plant and the experiment was replicated five times. Ivermectin (0.1ml/ml) was used as positive control. Among the plants, the crude extracts of P. dodecandrahad better activity that achieved maximum (100%) egg hatch inhibition at concentrations of 5 mg/ml while V. amygdalina and A. gummifera induced complete inhibition at concentration of 10 mg/ml after 48 hours of exposure. All the three plant crude extracts were inhibit egg hatchability significantly (p< 0.05) as compared  with the negative control but the inhibition among them were not significantly different in the effect. In conclusion, this study revealed that all of the three plant extracts have high inhibition potential on the hatchability of gastrointestinal nematode eggs. More detailed study on vivo anthelmintic effects of these plants with different extraction methods and phytochemical screening should be done.

Keywords: Albizia gummifera;Phytolacca dodecandra;Vernoniaamygdalina


Download data is not yet available.


1. Holm SA, Sörensen CR, Thamsborg SM, Enemark HL. Gastrointestinal nematodes and anthelmintic resistance in Danish goat herds. Parasite. 2014;21.
2. Perry BD, Randolph TF. Improving the assessment of the economic impact of parasitic diseases and of their control in production animals. Veterinary parasitology. 1999;84(3-4):145-68.
3. Central Stastical Agency. Livestock resources and production stastics in Ethiopia. Agricultural sample enumeration. Central Statistical Authority of the Federal Democratic Republic of Ethiopia. Addis Ababa, Ethiopia: CSA; 2008.
4. Perry BD., Randolph TF., McDermott JJ., Sones KR. and Thornton PK.Investing in animal health research to alleviate poverty. ILRI, Nairobi, Kenya. 2002.
5. Vatta AF, Letty BA, Van der Linde MJ, Van Wijk EF, Hansen JW, Krecek RC. Testing for clinical anaemia caused by Haemonchus spp. in goats farmed under resource-poor conditions in South Africa using an eye colour chart developed for sheep. Veterinary Parasitology. 2001;99(1):1-4.
6. Sutherland IA, Leathwick DM. Anthelmintic resistance in nematode parasites of cattle: a global issue?. Trends in parasitology. 2011;27(4):176-81.
7. Getachew S, Ibrahim N, Abebe B, Eguale T. In vitro evaluation of Anthelmintic activities of crude extracts of selected medicinal plants against Haemonchus contortus in AlemgenaWereda, Ethiopia. Acta ParasitologicaGlobalis. 2012;3:20-7.
8. Eguale T, Tilahun G, Gidey M, Mekonnen Y. In vitro anthelmintic activities of four Ethiopian medicinal plants against Haemonchus contortus. Pharmacologyonline. 2006;3:153-65.
9. Ministry of Agriculture(MoA). Budgeting and planning reports, summary of MoA, North Gondar zone, 2004.
10. Coles GC, Bauer C, Borgsteede FH, Geerts S, Klei TR, Taylor MA, Waller PJ. World Association for the Advancement of Veterinary Parasitology (WAAVP) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Veterinary parasitology. 1992;44(1-2):35-44.
11. Remison SU. Arable and vegetable crops of the tropics. Gift-Print Associates, Benin-City. 2005;45-50.
12. Biftu D, Nurfeta A, Jobre Y. Evaluation of anthelmintic activities of crude leaf extracts of three indigenous herbal plants against ovine gastrointestinal nematodosis.2004.
13. Chufamo B, Kechero Y, Bekele M,Beyene A. Comparison of the Efficacy of Different Modes of Extraction of 5 Tannin Rich Plants on Haemonchus contortus: Searching for Indicators Based on A Range of In vitro assays. Global Veterinaria.2013;11 (6):762-765.
14. Chufamo B, Kechero Y, Bekele M,Beyene A. Comparison of the Efficacy of Different Modes of Extraction of 5 Tannin Rich Plants on Haemonchus contortus: Searching for Indicators Based on A Range of In vitro assays. Global Veterinaria.2013;11 (6):762-765.
15. Mohammed A, Wossene A, Giday M, Tilahun G, Kebede N. In vitro anthelminthic activities of four medicinal plants against Haemonchus contortus. African Journal of Plant Science. 2013;7(8):369-73.
16. Innocent T, Deogracious O. The anthelmintic activity of selected indigenous medicinal plants used by The Banyankole of Western Uganda. Journal of animal and veterinary advances. 2006;5(8):712-7.
17. Nalule AS, Mbaria JM, Olila D, Kimenju JW. Ethnopharmacological practices in management of livestock helminthes by pastoral communities in the drylands of Uganda. Livestock Research for Rural Development. 2011;23(2):1-27.
18. Adediran OA, Uwalaka EC. Effectiveness evaluation of levamisole, albendazole, ivermectin, and Vernonia amygdalina in West African Dwarf goats. Journal of parasitology research. 2015: 1-5.
19. Alawa CB, Adamu AM, Gefu JO, Ajanusi OJ, Abdu PA, Chiezey NP, Alawa JN, Bowman DD. In vitro screening of two Nigerian medicinal plants (Vernonia amygdalina and Annona senegalensis) for anthelmintic activity. Veterinary Parasitology. 2003;113(1):73-81.
20. Sawleha Q, Dixit AK, Dixit P. Use of medicinal plants to control Haemonchus contortus infection in small ruminants. Veterinary World. 2010;3(11):515.
16 Views | 24 Downloads
Research Article