Evaluation of Recombinant Interleukin-2 on Humoral Immune Response of Newcastle Disease Vaccinated Chickens

Abstract

Totally 120 chickens belonging to three groups (group-A, B and C) were used to study the effect of Rec IL-2 on specific immunity of Newcastle disease vaccinated chickens. Serum samples collected at 6^th,12^th and 18^th days post vaccination (F strain and R₂B strain) were subjected to HI test and SRID test to assess the specific immunity of chickens.HI test results of chickens vaccinated with R₂B strain of NDV revealed the titre values in group-A showing 1:256,1:256 and 1:512 in the samples collected at 6^th ,12^th and 18^th days of post vaccination respectively. Whereas titre values in group-B were also in increasing trend, but remained at 1:256 titres. Group-C showed minimal titre values at 18^th days of post vaccination (1:4).SRID test results revealed the readings of antibodies levels in group-A after vaccination with R₂B strain, raised up to 4 mg/ml in 18^th day sampling, while group-C showed 2.5 mg/ml of antibodies even in 18^th day of sample collection indicating immunomodulatory effect of Rec IL-2 in vaccinated chickens.