Evaluation and detection of Brassicaceae leafy whey phytohormones efficacy for exploiting as a medium obtaining optimistic culture of fungi in vitro

  • Rajesh Khanduji Jadhav Head and Associate Professor, Department of Botany, D. G. Ruparel College, Mahim, University of Mumbai, Mumbai 400016, India


The  DPJ  (Deproteinised  Juice)  or  whey  constituents    were  responsible  for  the  induction  of  growth  optimization  of  plants,  various  fungi  including  yeast,  Rhizobium  reported  by  earlier  workers. In  previous  experiments,  DPJ  maximised  the  growth  of  plants  and  seed  germination. During  present  investigation  the  carbohydrates,  amino  acids  and  protein   tests  were  taken  into  the  consideration. All the tests found positive.  Despite,  the  extract is  deproteinised,  still  there  was  persistence  of  few  proteins  and  amino  acids. The  collection  of  mycelia  grown  on  DPJ  was  filtered  and    the  culture  filtrates    recommended  to   use  in vitro  for  the  industrial  purpose  for   biomass  and  secondary  metabolites.   Experimental  DPJ  is  compared  with  the  glucose  nitrate  medium  as  control.  These  positive  tests  revealed  the suitability  of  DPJ  to  be  used  as  the  medium  for  the  growth  of  fungi.  Positive  amino  acid  tests  conspicuously  revealed  presence  of  phytohormones  in  members  of   Brassicaceae  DPJ  and  hence  advisable  to  be  utilised  for  the   plant  growth  in  vivo,  plant  callus  growth   and  cell  proliferation  of  mycelia  in  vitro


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