TY - JOUR AU - Tata, Sape Subba PY - 2020/04/07 Y2 - 2024/03/29 TI - Development of a profused In vitro shoot multiplication using leaf explants of Bacopa monnieri (L.) Pennell JF - Current Botany JA - CB VL - 11 IS - 0 SE - Regular Articles DO - 10.25081/cb.2020.v11.6030 UR - https://updatepublishing.com/journal/index.php/cb/article/view/6030 SP - 14-17 AB - <p><em>Bacopa monnieri </em>(L.) Pennell is an important medicinal plant used for the preparation of medhyarasayan (rasayana). Leaf explants of field grown young plants of <em>B. monnieri </em>was used to establish an efficient regeneration protocol with cytokinin (BAP) and auxin (IAA). The highest multiplication, i.e. (220 shoots/leaf, a cumulative of 2200 shoots from 10 explants) were noticed after 45 days of culture in MS medium supplemented with BAP(1.5mg/L) and IAA(0.5mg/L). The optimum concentration of growth regulator for shoot elongation and rooting was recorded in MS+GA<sub>3</sub>(0.25mg/L) and MS+IBA(1.5mg/L) respectively. The rooted plantlets were successfully established in green house conditions.</p> ER -