Screening and Biochemical Characterization of Wheat Cultivars Resistance to Magnaporthe oryzae pv Triticum (MoT)
DOI:
https://doi.org/10.25081/jpsp.2020.v6.6096Keywords:
Wheat, Magnaporthe oryzae pv. Triticum, Resistance, Antioxidant, Oxidative stressAbstract
Global food security is seriously threatened due to increased frequency and occurrence of fungal diseases. One example is wheat blast caused by Magnaporthe oryzae is a fungal diseases of rice, wheat, and other grasses, that can destroy the whole food production to sustain millions of people. Wheat blast was first detected in february 2016 with a serious outbreak in Asia. Assessment of the available germplasms to stress tolerant/resistant is one of the best options for developing stress tolerant crop varieties. In this study, a total of sixteen wheat cultivars were collected and test their disease severity to blast pathogen Magnaporthe oryzae pv. Triticum (MoT). Among the varieties, BARI Gom 33 exhibited partially resistance against blast pathogen, whereas all other genotypes become susceptible to MoT. Different yield and yield contributing characters of both resistant and susceptible cultivars were also evaluated and found no significant differences among them. To understand the underlying mechanism of resistance in BARI Gom 33, antioxidant enzyme activity, concentration of reactive oxygen species and cellular damage after fungal infection were also evaluated and found that activities of ascorbate peroxidase (APX), catalase (CAT) and peroxidase (POD) were higher in BARI Gom 33 than BARI Gom 25 and BARI Gom 31. The hydrogen peroxide (H2O2) and malondealdehyde (MDA) content in BARI Gom 33 was low compare to BARI Gom 25 and BARI Gom 31, which may due to greater increase of the APX, CAT and POD in resistant genotypes. Thus, it may suggest that a more efficient antioxidative defense system in BARI Gom 33 during the infection process of M. oryzae restricts the cell damage caused by the fungus. The identified genotypes can either be used directly in the blast prone area or as a source of resistance to further development of blast resistance high yielding wheat variety.
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References
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33. Ou S H. Rice Diseases. Commonwealth Mycological Institute, Kew, UK. 1985.
34. Fu J, and Huang B. Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environ. Exp. Bot. 2001; 45:105-114.
35. Kuzniak E, and Sklodowska M. Fungal pathogen-induced changes in the antioxidant systems of leaf peroxisomes from infected tomato plants. Planta. 2005; 222:192-200.
36. Xavier Filha M S, Rodrigues F A, Domiciano G P, Oliveira H V, Silveira P R and Moreira W R. Wheat resistance to leaf blast mediated by silicon. Australas. Plant Pathol. 2011; 40:28-38.
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2. FAOSTAT Data, Food and agricultural commodities production. Rome, Italy: 2012. http://faostat.fao.org .
3. Shewry PR, Wheat. Journal of Experimental Botany. 2009; 60 (6): 1537–1553.
4. Rosegrant MW, Agcaoili M. Global food demand, supply, and price prospects to 2010. Washington, DC: 2010.
5. Malaker PK, Barma NC, Tiwary TP, Collis WJ, Duveiller EP, Singh K, Joshi AK, Singh RP, Braun HJ, Peterson GL, Pedley KF. First report of wheat blast caused by Magnaporthe oryzae pathotype triticum in Bangladesh. Plant Disease. 2016; 100(11):2330.
6. Igarashi S. Update on wheat blast (Pyricularia oryzae) in Brazil. Proceedings of the International Conference-Wheat for the Nontraditional Warm Areas, CIMMYT, Mexico. 1990. 480- 483.
7. Islam MT, Croll D, Gladieux P, Soanes DM, Persoons A, Bhattacharjee P, Hossain MS, Gupta DR, Rahman MM and Mahboob MG. Emergence of wheat blast in Bangladesh was caused by a South American lineage of Magnaporthe oryzae. BMC Biology. 2016; 14:84. https://doi.org/10.1186/s12915-016-0309-7
8. Farman M, Peterson G, Chen L, Starnes J, Valent B, Bachi P, Murdock L, Hershman D, Pedley K, Fernandes JM, Bavaresco J. The Lolium pathotype of Magnaporthe oryzae recovered from a single blasted wheat plant in the United States. Plant Disease. 2017 May 13; 101(5):684-92.
9. Goulart A, and Paiva F. Perdas no rendimiento de grãos de trigo causada por Pyricularia grisea, nos anos de 1991 e 1992, no Mato Grosso do Sul. Sum. Phytopathol. 2000; 26:279-282.
10. Urashima AS, Bockus WW, Bowden RL, Hunger RM, Morrill WL, Murray TD, Smiley RW (eds). Compendium of wheat diseases and pests. Saint Paul, MN: American Phytopathological Society.2010; pp. 22–23.
11. Arruda M A, Bueno C R N C, Zamprogno K C, Lavorenti NA, and Urashima A. Reação do trigo a Magnaporthe grisea nos diferentes estádios de desenvolvimento. Fitopatol. 2005; 30:121-126.
12. Cruz M F A, Prestes A M, Maciel J L N, and Scheeren P L. Resistência parcial à brusone de genótipos de trigo comum e sintético nos estádios de planta jovem e de planta adulta. Trop. Plant Pathol. 2010; 35:24-31
13. Caverzan A, Casassola A, Brammer SP. Antioxidant responses of wheat plants under stress. Genetics and molecular biology. 2016 Mar; 39(1):1-6.
14. Mullineaux P M, & Baker N R. Oxidative stress: antagonistic signaling for acclimation or cell death? Plant physiology. 2010; 154(2):521-525.
15. Sharma P, Jha A B, Dubey R S, & Pessarakli M. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of botany. 2012.
16. Wang ZY, Li FM, Xiong YC and Xu BC. Soil-water threshold range of chemical signals and drought tolerance was mediated by ROS homeostasis in winter wheat during progressive soil drying. J Plant Growth Regul. 2008; 27:309-319.
17. Varga B, Janda T, Laszlo E and Veisz O. Influence of abiotic stresses on the antioxidant enzyme activity of cereals. Acta Physiol Plant. 2012; 34:849-858.
18. Huseynova IM, Aliyeva DR and Aliyev JA. Subcellular localization and responses of superoxide dismutase isoforms in local wheat varieties subjected to continuous soil drought. Plant Physiol Biochem. 2014; 81:54-60.
19. Kong L, Wang F, Si J, Feng B, Zhang B, Li S and Wang Z. Increasing in ROS levels and callose deposition in peduncle vascular bundles of wheat (Triticum aestivum L.) grown under nitrogen deficiency. J Plant Interact. 2014; 8:109-116.
20. Talaat NB and Shawky BT. Modulation of the ROSscavenging system in salt-stressed wheat plants inoculated with arbuscular mycorrhizal fungi. J Plant Nutr Soil Sci. 2014; 177:199-207.
21. Debona D, Rodrigues F A, Rios J A, Nascimento K J T, Silva L C. The effect of silicon on antioxidant metabolism of wheat leaves infected by Pyricularia oryzae. Plant pathology. 2014; 63(3):581-589.
22. Agrawal G K, Jwa N S, Rakwal R. A pathogen-induced novel rice (Oryza sativa L.) gene encodes a putative protein homologous to type II glutathione-S-transferases. P Sci. 2002; 163:1153-1160.
23. Agrawal G K, Rakwal R, Jwa N S & Agrawal V P. Effects of signaling molecules, protein phosphatase inhibitors and blast pathogen (Magnaporthe grisea) on the mRNA level of a rice (Oryza sativa L.) phospholipid hydroperoxide glutathione peroxidase (OsPHGPX) gene in seedling leaves. Gene. 2002; 283(1-2):227-236.
24. IRRI. Standard Evaluation System for Rice. International Rice Research Institute, Los Banos, Philippines. 2014.
25. Aebi H. Catalase in vitro. In Methods in enzymology. Academic Press. 1984; 105:121-126.
26. Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 1981; 22:867–880.
27. Heath RL, Packer L. Photoperoxidation in isolated chloroplasts. I-kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics. 1968; 125: 189–198.
28. Velikova V, Yordancv I, Edreva A. Oxidative stress and some antioxidant systems in acid rain-treated bean plants: protective role of exogenous polyamines. Plant Science. 2000; 151:59-66.
29. Farhad M, Velu G, Hakim MA, Kabir MR, Alam MA, Mandal MS, Hossain A, Reza MA, Mustarin K, Jahan, AHS, Barma NCD. Development and deployment of Biofortified and blast resistant wheat variety in Bangladesh. 2018. Poster. BARI research progress. https://www.researchgate.net/publication/330073588
30. Magbanua Z V, De Moraes C M, Brooks T D, Williams W P, and Luthe D S. Is catalase activity one of the factors associated with maize resistance to Aspergillus flavus? Mol. Plant-Microbe Interact. 2007; 20:697-706.
31. Rauyaree P, Choi W, Fang E, Blackmon B and Dean R. Genes expressed during early stages of rice infection with the rice blast fungus Magnaporthe grisea. Mol. Plant Pathol. 2001; 2:347-354.
32. Mandal S, Mitra A, and Mallick N. Biochemical characterization of oxidative burst during interaction between Solanum lycopersicum and Fusarium oxysporum f. sp. lycopersici. Physiol. Mol. Plant Pathol. 2008; 72:56-61.
33. Ou S H. Rice Diseases. Commonwealth Mycological Institute, Kew, UK. 1985.
34. Fu J, and Huang B. Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environ. Exp. Bot. 2001; 45:105-114.
35. Kuzniak E, and Sklodowska M. Fungal pathogen-induced changes in the antioxidant systems of leaf peroxisomes from infected tomato plants. Planta. 2005; 222:192-200.
36. Xavier Filha M S, Rodrigues F A, Domiciano G P, Oliveira H V, Silveira P R and Moreira W R. Wheat resistance to leaf blast mediated by silicon. Australas. Plant Pathol. 2011; 40:28-38.
37. Quan L J, Zhang B, Shi W W and Li H Y. Hydrogen peroxide in plants: a versatile molecule of the reactive oxygen species network. J. Integr. Plant Biol. 2008; 50:2-18.
38. El-Zahabi H M, Gullner G, and Királi Z. Effects of powdery mildew infection of barley on the ascorbate-glutathione cycle and other antioxidants in different host–pathogen interactions. Phytopathology. 1995; 85:1225-1230.
39. Hückelhoven R, Dechert C, Trujillo M and Kogel K H. Differential expression of putative cell death regulator genes in nearisogenic, resistant and susceptible barley lines during interaction with the powdery mildew fungus. Plant Mol. Biol. 2001; 47:739-748.
Published
05-04-2020
How to Cite
Biswas, C. S., A. Hannan, A. Monsur, and G. H. M. Sagor. “Screening and Biochemical Characterization of Wheat Cultivars Resistance to Magnaporthe Oryzae Pv Triticum (MoT)”. Journal of Plant Stress Physiology, vol. 6, Apr. 2020, pp. 1-6, doi:10.25081/jpsp.2020.v6.6096.
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Research Article
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