Combined seed enhancement technique involving seed priming and coating for improvised anatomical potential and vigour of okra seeds (Abelmoschus esculentus L.)

  • Anilkumar L Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore - 641 003, Tamil Nadu, India
  • Malarkodi K Agricultural Research Station, Bhavanisagar, Tamil Nadu Agricultural University, Tamil Nadu, India


            The study was conducted to standardize the methodology of humid priming for improving the seed germination and vigour of okra seeds and to compare the efficacy of different enhancement techniques such as hydro priming, spin priming, seed coating and the combination of ‘humid priming + seed coating’ in improving the seed vigour characteristics of okra (Abelmoschus esculentus L.). In order to standardize the humid priming technique, okra seeds were subjected to humid priming for four different duration’s viz., 2, 4, 6 and 8 h, shade dried to original moisture content and subjected to evaluation of seed germination   under shade net conditions in raised beds. The results revealed that 4 h of humid priming was statistically significant to the rest of the durations, with respect seed germination and seedling growth. With respect to anatomical potential, the radicle length (3.39mm), cotyledon length (6.69mm) and cotyledon width (6.30mm), recorded by humid priming (4 h) was 62.2 %, 6.4 % and 2.6 % higher than control seeds. The second experiment conducted to compare the efficacy of hydro priming, spin priming, polymer coating and combination of humid priming + seed coating. The study revealed that seeds subjected to combination of humid priming (4 h) + seed coating (3g kg-1) recorded highest physiological potential in terms of germination percentage (87 %), seedling length (20.2 cm), dry matter production (421.3 mg), vigour index (1765), root volume (3.5 cm3) and root sheath (2.560 g) while the control recorded only 70 %, 15.1 cm, 328.7 mg, 1055, 2.0 cm3 and 1.532 g, respectively.

Keywords: Okra; seed priming techniques; humid priming; seed anatomical potential; seed germination; seedling vigour


Download data is not yet available.


1. Leela P, Rekha GS, Arumugam K. Allelopathic potential of Echinochloa crusgalli (l.) extracts on growth and developmental changes of Abelmoschus esculentus (l.) and Solanum lycopersicum (L.). Journal of Plant Stress Physiology. 2018:33-37.
2. 2018
3. Elangovan R, Pavadai P. Gamma rays, EMS and DES induced changes on cytology of bhendi [Abelmoschus esculentus (L.) Moench]. Journal of Scientific Agriculture. 2017;1:19-24.
4. Anitha S, Karthika N. Regular article antixenosis resistance in okra accessions against shoot and fruit borer Earias vittella (fab.). Journal of Phytology. 2018;10:27-32.
5. Kumar A, Puniya RC, Bhuker A, Sharma R, Devi R. Seed quality as influenced by seed invigouration treatments in pigeonpea [Cajanus cajan (L.) Millsp.]. Legume Research: An International Journal. 2017;40.
6. Basu RN. An appraisal of research on wet and dry physiological seed treatments and their applicability with special reference to tropical and sub-tropical countries. Seed Science and Technology. 1994;22:107-126.
7. Heydecker W, Coolbear P. Seed treatments for improved performance survey and attempted prognosis. Seed science and Technology. 1977.
8. Bradford KJ. Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. Horti Science (USA). 1986.
9. Nawaz J, Hussain M, Jabbar A, Nadeem GA, Sajid M, Subtain MU, Shabbir I. Seed priming a technique. International Journal of Agriculture and Crop Sciences. 2013;6:1373.
10. Mohamed M. Assessment of factors influencing seed propagation of Melia dubia Cav. (M. Sc.(Forestery)), TamilNadu Agricultural University, Coimbatore. (2014).
11. Powell AA, Yule LJ, Jing HC, Groot SP, Bino RJ, Pritchard HW. The influence of aerated hydration seed treatment on seed longevity as assessed by the viability equations. Journal of Experimental Botany. 2000;51:2031-2043.
12. Nath S, Coolbear P, Hampton JG. Hydration-dehydration treatments to protect or repair stored ‘Karamu’wheat seeds. Crop Science. 1991;31:822-826.
13. Loupassaki M, Vasilakakis M, Androulakis I. Effect of pre-incubation humidity and temperature treatment on the in vitro germination of avocado pollen grains. Euphytica. 1997;94:247-251.
14. Cohen E, Lavi U, Spiegel-Roy P. Papaya pollen viability and storage. Scientia Horticulturae. 1989;40:317-324.
15. Luza JG, Polito VS, Weinbaum SA. Staminate bloom date and temperature responses of pollen germination and tube growth in two walnut (Juglans) species. American Journal of Botany. 1987;74:1898-1903.
16. Henny RJ. Relative humidity affects in vivo pollen germination and seed production in Dieffenbachia maculate 'Perfection'. Journal of the American Society for Horticultural Science. 1980;105:546-548
17. Henny RJ. Pollen germination in aglaonema flowers of different ages. HortScience (USA). 1988.
18. Simons DH, Sfakiotakis E, Dilley DR. Enhancement of in vitro pollen germiantion of lily with increased pre-inoculation humidity. HortScience. 1972.
19. Van Ravestijn W. Aanleg bloemen en verbeteren zetting bij paprika (Flower induction and improvement of fruit set in Capsicum). Groenten Fruit. 1986;41:32-35 (in Dutch).
20. Lee TA, Ketring DL, Powell RD. Flowering and growth response of peanut plants (Arachis hypogaea L. var. Starr) at two levels of relative humidity. Plant physiology. 1972;49:190-193.
21. Khan MM, Qasim M, Iqbal MJ, Naeem AF, Abbas M. Effect of seed humidification on germinability, vigour and leakage in cockscomb (Celosia argentia var. cristata L.). International Journal of Agriculture and Biology. 2003;5:499-503.
22. Thornton JM, Collins AR, Powell AA. The effect of aerated hydration on DNA synthesis in embryos of Brassica oleracea L. Seed Science Research. 1993;3:195-199.
23. Vijayalakshmi K, Sundaralingam K. Enhancement of Vigour Status through Micronutrient Priming and Humid Invigouration in Sesame. Madras Agricultural Journal. 2018;105:40-43.
24. Karssen CM. Two sites of hormonal action during germination of Chenopodium album seeds. Physiologia plantarum. 1976;36:264-270.
25. Pill WG, Haynes JG. Gibberellic acid during priming of Echinacea purpurea (L.) Moench seeds improves performance after seed storage. Journal of Horticultural Science. 1996;71:287-295.
26. Nirmala K, Umarani R. Evaluation of seed priming methods to improve seed vigour of okra (Abelmoschus esculentus) and beetroot (Beta vulgaris). Seed Science and Technology. 2008;36:56-65.
27. Chandan MN, Malarkodi K, Umarani R. Evaluation of anatomical potential of primed and non- primed seeds of solanaceous vegetables. Journal of Pharmacognosy and Phytochemistry SP4. 2018;174-178.
28. Karthi P. Development of hormone based seed coating formulation to improve seed germination and seedling vigour of crop seeds. M. Sc.(Ag.) Thesis, Depatment of Seed Science and Technology, TamilNadu Agricultural University, Coimbatore. 2017
29. Maguire JD. Speed of Germination-Aid in Selection and Evaluation for Seedling Emergence and Vigor 1. Crop science. 1962;2:176-177.
30. Abdul-Baki AA, Anderson JD. Vigor determination in soybean seed by multiple criteria 1. Crop science. 1973;13:630-633.
31. Austin R, Longden PC, Hutchinson J. Some effects of ‘hardening’ of carrot seed. Annals of Botany. 1969;33:883-895.
32. Sivritepe HO, Dourado AM. The effects of humidification treatments on viability and the accumulation of chromosomal aberrations in pea seeds. Seed science and technology. 1994;22:337-348.
33. Umarani R, Bhaskaran M, Santhiya R. Nutrient based seed coating formulation for enhancement of seed germination characteristics, crop growth and productivity of cotton. International Journal of Current Microbiology and Applied Science. 2017;6:5429-5438.
34. Hartmann HT, Kester DE. Davies Junior FT. Geneve RL. Plant propagation: principles and practices. 2002.
35. Baskin JM, Baskin CC. A classification system for seed dormancy. Seed science research. 2004;14:1-6
36. Taiz E, Zeiger L. Fisiologia vegetal. 3.ed. Porto Alegre: Artmed, 2004. pp719.
119 Views | 118 Downloads
How to Cite
L, A., and M. K. “Combined Seed Enhancement Technique Involving Seed Priming and Coating for Improvised Anatomical Potential and Vigour of Okra Seeds (Abelmoschus Esculentus L.)”. Journal of Phytology, Vol. 11, no. 1,  , pp. 25-30, doi:
Research Article