Horticultural Biotechnology Research http://updatepublishing.com/journal/index.php/hbr <p>The <strong>Horticultural Biotechnology Research </strong>is a peer-reviewed, open access journal that publishes articles in all the in the fields of Horticultural Biotechnology.</p><p><strong>Submission</strong></p><p>The journal welcomes the submission of original manuscripts (Please see <a href="/plagiarism-policy.php">Plagiarism Policy</a>) that meet the general criteria of significance and scientific excellence. The journal publishes Review Articles, Regular Articles and Short Communications. For detailed guide for preparation of manuscript please visit <a href="/guide-for-authors.php">Author’s Guide</a> and for submitting, please sign in the <a href="/journals/index.php/cb/about/submissions#onlineSubmissions">Online Submission</a> page. If the author experiences any difficulties during online submission, contact hbr@scienceflora.org for support.</p><p><strong>Peer Review</strong></p><p>Soon after submission, the article will be screened for plagiarism checking with iThenticate software. The articles those passed the iThenticate screening, will be peer-reviewed with two external peer reviewers. After revision, before final acceptance, the article will be again screened for plagiarism. The acceptance criteria for all papers are the quality and originality of the research, its significance to the scientific community and interest to a wide audience.</p><p><strong>Frequency</strong></p><p>Horticultural Biotechnology Research is a bimonthly journal (6 issues per volume per year), but the articles will be published immediately after proof corrections, means; the articles will be added to the current issues continuously without any delay. This helps the journal in rapid publication of accepted articles.</p><p>Manuscripts will be published online as soon as the authors approved the final proof. Therefore, there will not be a delay period for accepted articles to be published online.</p> Scienceflora Publishers Pvt. Ltd. en-US Horticultural Biotechnology Research 2455-460X CHARACTERIZATION OF SOME ALGERIANS AND FOREIGN LENTIL ACCESSIONS BY QUALITATIVE TRAITS http://updatepublishing.com/journal/index.php/hbr/article/view/3560 <p>In the present study an attempt has been made to characterize lentil accessions based on qualitative traits. There were variations among 44 lentil accessions. Erect growth habit was observed in 24 % of the accessions where 8% were from Algeria. Conversely, prostrate growth habit was observed in 34% of the accessions. The majority of the remaining accessions (41.32%) were intermediate. Stem with anthocyanin pigmentation was showed in 44% of the accessions, whereas, 56% had no pigmentation (green stem). About half of the accessions had grey green leaves (53%) and 23% of accessions were light green. Among the characters, flower color showed the highest variation. White flowers were observed in 64% accessions and violet flowers were found in 36% accessions. Flowers, with violet stripes in the standard petal (SVE) were observed in 44% accessions and the majority (56%) lacked violet stripes. Yellow cotyledons were observed in 61% accessions, while the rest (39%) had red cotyledons. The majority of accessions (81%) were observed with brown testa while 14% were green and 5% had yellow testa. Absence of seed coat pattern was observed in 69% accessions. However, 8% accessions with spots, 5% with dots, 16% were marbled and the remaining 2% were complex. Flattened seed shape was observed in 60% of accessions. Conversely, globose shape was observed in 40% of the accessions, among of them 27% were from Algeria.</p> DJOUHER GAAD, MERIEM LAOUAR FATIMA GABOUN AISSA ABDELGUERFI ##submission.copyrightStatement## 2018-04-07 2018-04-07 43 47 10.25081/hbr.2018.v4.3560 Efficient in vitro organogenesis and plantlets regeneration in sesame (Sesamum indicum L.)-an important oilseed crop http://updatepublishing.com/journal/index.php/hbr/article/view/3462 <p>The regeneration methods in Sesame (<em>Sesamum indicum</em> L.) already developed were genotype dependent and were unsuccessful for Indian cultivars. Hence, an efficient protocol for <em>in vitro</em> organogenesis and plant regeneration in sesame was attempted with TMV 3 cultivar. Immature cotyledons derived from 1-week old seedlings were cultured on MS (Murashige and Shoog) medium fortified with different concentrations of 6-benzylaminopurine (BAP), thidiazuran (TDZ) and kinetin for adventitious shoot induction. It was found that optimal medium for direct shoot formation was MS with BAP (2.0 mg/l) at a frequency of 74% with an average of 4.5 shoots per expalnt. The shoot regeneration frequency was significantly reduced with either TDZ or kinetin when compared with BAP alone. Elongated individual shoots were transferred on MS media supplemented with Indole Butyric Acid (IBA; 0.5 mg/l) showed rooting frequency of 70%. The rooted plantlets were acclimatized to potting mixture containing sand, soil and clay mixture and grown to maturity with survival rate of 65 %. No phenotypic aberrations were observed among the <em>ex vitro</em> transferred plantlets. The protocol described here assures a high frequency of shoot regeneration, root induction and also plant survival rate.</p> R. Anandan, K.V. Deepak T. Deenathayalan M. Vignesh, B. Priyadharshini S. Murugan, M. Prakash ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2018-11-15 2018-11-15 01 05 10.25081/hbr.2018.v4.3462 Estimation of morphological and molecular genetic diversity in blackgram [Vigna mungo (L.) Hepper] under YMV hotspot regime http://updatepublishing.com/journal/index.php/hbr/article/view/3463 <p>A phenotypic and molecular diversity study was conducted using seven traits and 19 SSR markers in a collection of 26 black gram genotypes. Phenotypic characterization was based on seven yield and yield related variable. The &nbsp;field experiment &nbsp;was &nbsp;laid &nbsp;out &nbsp;at &nbsp;Panboli village (YMV hotspot) &nbsp;of Tirunelveli District in Tamilnadu during summer 2017. Genetic divergence was estimated on the basis of D<sup>2 </sup>values and 26 genotypes under study were grouped into six clusters by Tocher’s method. Seed yield per plant followed by Plant height and number of pods per plant contributed to the genetic divergence. The genetic distance announced using DICE dissimilarity co-efficient indicated highest divergence of 1.0 between VBN 8 and AUBG 17 and between VBN 8 and AUBG 19. The dendogram constructed using the DICE dissimilarity co-efficient between genotypes showed four apparent clusters based on marker allele distribution. Divergence was noted between the dissimilarity matrices based on the molecular and phenotypic diversity based on agronomic data.&nbsp;</p> S. Jeevitha, R. Karthikeyan, M. Vignesh A. Malarkodi, R. Tirumalai, A. J. Nainu R. Anandan, M. Prakash, S. Murugan ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2018-11-15 2018-11-15 06 09 10.25081/hbr.2018.v4.3463 Heterotic expression in inbreds derived from four different base populations in maize (Zea mays L.). http://updatepublishing.com/journal/index.php/hbr/article/view/3464 <p>The concepts of combining ability and heterosis are the fundamental tools for enhancing productivity of different crops. The main objective is to study the usefulness of inbreds derived from four different base populations namely advanced generations of single cross hybrids, three way cross hybrids, hybrid mixtures and composites by analyzing the combining ability of inbreds and crosses derived and the heterosis obtained for important characters. One hundred and forty four maize inbreds derived from four different base populations namely advanced generations of single cross hybrids, three way cross hybrids, hybrid mixtures and composites were studied for their heterotic expression. The results indicated that composite and hybrid mixture base populations shall be of great use in deriving genetically divergent inbreds and single cross hybrids with significant standard heterosis suitable for commercial exploitation.</p> B. Bharathiveeramani M. Prakash A. Seetharam ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2018-11-15 2018-11-15 10 15 10.25081/hbr.2018.v4.3464 UTILIZATION OF CONVENTIONAL AND NON-CONVENTIONAL ORGANIC SOURCES AND INDUSTRIAL BY-PRODUCTS FOR YIELD MAXIMIZATION OF BRINJAL IN COASTAL SOIL http://updatepublishing.com/journal/index.php/hbr/article/view/3479 <p>This study was conducted to evaluate the response of brinjal (<em>Solanum melongena </em>L.) with conventional, non-conventional organic source, industrial by-products combined with inorganic fertilizers. The treatments imposed were T<sub>1</sub> – Control (100% RDF), T<sub>2</sub>-100% RDF + Municipal Solid Waste Compost <br> @ 5 t ha<sup>-1</sup>, T<sub>3</sub> – 100% RDF + Municipal Solid Waste Compost @ 10 t ha<sup>-1</sup>, T<sub>­4</sub> – 100% RDF + Vermicompost @ 2.5 t ha<sup>-1</sup>, T<sub>5</sub> – 100% RDF + Vermicompost @ 5 t ha<sup>-1</sup>, <br> T<sub>6</sub> – 100% RDF + Rice Husk Ash @ 5 t ha<sup>-1</sup>, T<sub>7</sub> – 100% RDF + Rice Husk Ash @ <br> 10 t ha<sup>-1</sup>, T<sub>8</sub> – 100% RDF + Lignite Fly Ash @ 5 t ha<sup>-1</sup>, T<sub>9</sub> – 100% RDF + Lignite Fly Ash @ 10 t ha<sup>-1</sup>. There were nine treatments combinations replicated thrice in CRD. The soil was sandy in texture with available nitrogen, phosphorus and potassium of 257, 10.2 and 117 kg ha<sup>-1</sup> respectively which fell in fertility status of low. The soil classified taxonomically as <em>Typic Udisamments. </em>The results showed that application of 100% RDF + Vermicompost @ 5 t ha<sup>-1</sup> (T<sub>5</sub>) significantly increased yield attributes <em>viz., </em>fruit length (16.6 cm), fruit girth (15.2 cm), no. of fruits plant<sup>-1</sup> (20.67), fruit weight (45.2 g) and fruit yield plant<sup>-1</sup> (934.2 g plant<sup>-1</sup>). This treatment registered maximum quality attributes like ascorbic acid content (4.5 mg 100 g<sup>-1</sup>), crude protein (4.81%), drymatter of fruit (70.2 g plant<sup>-1</sup>) and dry matter of plant (81.3 g plant<sup>-1</sup>). The post harvest soil available N (148mgkg-1) and P (7.15mgkg-1)status was higher due to application of 100% RDF with Vermicompst @ 5 t ha<sup>-1</sup> (T<sub>5</sub>).&nbsp; The post harvest available K (75.58mgkg-1) status recorded higher in the treatment T<sub>9</sub> which received 100% RDF with Lignite Fly Ash @ 10 t ha<sup>-1</sup> (T<sub>9</sub>).</p> D. Venkatakrishnan ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2018-11-15 2018-11-15 16 18 10.25081/hbr.2018.v4.3479 EFFECT OF CHICORY LEAF EXTRACT SEED HARDENING CUM FOLIAR SPRAY TO IMPROVE RESULTANT SEED QULAITY IN SESAME (Sesamum indicum L.) cv. TMV 3 UNDER RAINFED CONDITION. http://updatepublishing.com/journal/index.php/hbr/article/view/3482 <p>An evaluation was carried out to study the effect pre sowing seed treatment and foliar spay with chicory leaf extract on resultant seed quality characters of sesame cv TMV 3. The bulk seeds were graded for uniformity using appropriate round perforated metal sieves of sizes of 5/64" size sieve and were imposed various concentration of chicory leaf extract presowing treatment coupled with foliar spray at different concentration under drought condition. After harvest the resultant seeds were evaluated for its seed qualities. The results revealed that the that the Chicory @ 20 % Pre sowing seed treatment + Chicory foliar spray @ 30 %&nbsp; during pod filling stage&nbsp; significantly increased the resultant seed quality characters of the sesame, when compared to other concentration and control.</p> G. Sathiya Narayanan, B. Sunil Kumar M. Prakash, R. Anandan ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2018-11-15 2018-11-15 19 22 10.25081/hbr.2018.v4.3482 Studies on effect of induced mutagenesis on Finger millet (Eleusine coracana (L.) Gaertn.) Var- CO 13 in M1 generation http://updatepublishing.com/journal/index.php/hbr/article/view/3485 <p>The present study was carried out to induce chemical mutagens in Finger millet (<em>Eleusinecoracana</em>(L.) Gaertn.). The seed were subjected to different treatment level of EMS and DES. The parameters like Plant height(cm), Number of leaves per plant, Leaf length (cm/plant), Number of&nbsp; finger per plant, Finger length (cm/plant), Days to first bloom, Yield per plant (g) and 1000 grains weight (g)were observed in M<sub>1</sub>generation. And the result revealed that, all the parameters except days to first blooming show a dose dependent decrease in both treatments. The LD-<sub>50</sub> value was found in 30mM of EMS and 40mM of DES.</p> K. Aviya, L. Mullainathan ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2018-11-15 2018-11-15 23 25 10.25081/hbr.2018.v4.3485 HETEROSIS FOR YIELD AND YIELD ATTRIBUTED TRAITS IN RICE (Oryza sativa L.) http://updatepublishing.com/journal/index.php/hbr/article/view/3490 <p>Rice is the foremost food crop among cereals and it is staple food crop providing high calories for the people of South East Asia. The Objectives of the study was the extent of heterosis components of grain yield in F1 hybrids. The Materials for this study considered of parents and thirty F1 hybrids derived from crossing of three cross lines <em>viz.,</em> IR58025A, IR62829A and PUSA3A and ten testers. Pollen and spikelet fertility revealed the identification of ten testers. For heterosis studies majority of the crosses showed positive heterosis for the characters <em>viz.,</em> productive tillers, filled grains per panicle, spikelet fertility, thousand grain weight, grain yield per plant, grain length, grain breadth, grain L/B ratio, kernel L/B ratio, milling recovery, head rice recovery, and amylose content. Negative heterosis were recorded for the characters namely days to first flowering, plant height, panicle length, kernel length, kernel breadth and alkali digestion value. Hybrids IR58025A ´ ASD19, IR62829A ´ ASD16, PUSA3A ´ IR42 were found to record high per se performance, higher percentage of standard heterosis for majority of the traits.</p> J. GOKULAKRISHNAN ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2018-11-15 2018-11-15 26 29 10.25081/hbr.2018.v4.3490 INFLUENCE OF WEATHER FACTORS ON INCIDENCE OF SHOOT AND FRUIT BORER (Earias vittella Fabiricus) ON BHENDI http://updatepublishing.com/journal/index.php/hbr/article/view/3491 <p>The field experiments were carried out in the farmer field at C. Mutlur near Chidambaram during rabi and kharif 2017. The results of experiments revealed that the per cent shoot damage and fruit damage by <em>E. vittella </em>on bhendi &nbsp;reached peak on 13<sup>th</sup> and 15<sup>th</sup> MSW in rabi season, whereas 43<sup>rd</sup> and 42<sup>nd</sup> MSW in kharif season. The per cent shoot damage and fruit damage by <em>E. vittella </em>were exerted significantly positively correlated with minimum (0.552 &amp; 0.698) and maximum temperature (0.629 &amp; 0.748), whereas rainfall positively correlated with per cent shoot and fruit damage but it was non significant (0.111 &amp; 0.297), however relative humidity (-0.178 &amp; -0.210) and sunshine hours were exerted negatively correlated with per cent shoot damage and fruit damage by <em>E. vittella</em> during rabi 2017. The kharif season 2017 indicated that per cent shoot damage and fruit damage of bhendi was positively non significant association with RH and rain fall, while negatively non significant correlation with minimum temperature (-0.43 &amp; -0.309) and sunshine hours (-0.265 &amp; -0.283) was recorded.</p> K. ARCHUNAN, M. PAZHANISAMY, S. SATHYA ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2018-11-15 2018-11-15 30 34 10.25081/hbr.2018.v4.3491 STUDIES ON HETEROSIS FOR YIELD IN BRINJAL (Solanum melongena. L) http://updatepublishing.com/journal/index.php/hbr/article/view/3492 <p>Heterosis breeding is one of the most efficient tools to exploit the heterotic response for various useful traits. The Line × Tester mating design was undertaken with seven lines and three testers were evaluated along with twenty one hybrids in randomized block design to estimate the magnitude of heterosis for yield and its yield contributing characters. The best way to utilize heterosis in crop is to generate F<sub>1</sub> hybrids having maximum heterozygocity, thereby facilitating the identification and selection of hybrid vigor. The positive significant standard heterosis for fruit yield per plant was maximum with L<sub>3</sub> × T<sub>1</sub> (28.94%) followed by L<sub>7</sub> × T<sub>3</sub> (15.73%) and L<sub>7</sub> × T<sub>1 </sub>(15.10%) respectively. Some of the promising hybrids have showed desirable heterosis for plant height, number of fruits per plant,1000 seed weight, seedling shoot length, seedling root length and seedling dry weight.</p> G. KALAIYARASI, S. RANJITH RAJA RAM, K. R. SARAVANAN ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2018-11-15 2018-11-15 35 38 10.25081/hbr.2018.v4.3492 STUDIES ON GENETIC DIVERSITY IN Vigna mungo L. Hepper IN YMV HOTSPOT http://updatepublishing.com/journal/index.php/hbr/article/view/3493 <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; The present investigation was conducted to examine the 41 blackgram genotypes&nbsp; along with one check (T-9) to study the genetic diversity. Analysis of variance showed highly significant differences among 41blackgram genotypes for 9 quantitative characters studied. Maximum genotypic and phenotypic variance was recorded for percentage of disease infection, single plant seed yield, and number of pods per plant.&nbsp;&nbsp; Minimum&nbsp;&nbsp; GCV and PCV were recorded for pod length, days to 50% flowering, number of seeds per pod. High heritability was recorded for percentage of disease infection, single plant seed yield, and number of pods per plant. High heritability coupled with high genetic advance as percent of mean was recorded for percentage of disease infection, single plant seed yield. Genetic diversity estimated in 41 blackgram genotypes using Mahalanobis’s D<sup>2 </sup>statistic. Forty one genotypes were grouped into seven clusters by Tocher method (Mahalanobis Euclidean Distance) cluster analysis.&nbsp; The&nbsp; maximum&nbsp; inter-cluster&nbsp; distance&nbsp; was&nbsp; observed&nbsp; between&nbsp; cluster VI&nbsp; and&nbsp; cluster&nbsp; VII and maximum intra-cluster distance was observed in cluster VI.&nbsp; Cluster VII showed maximum cluster mean value for seed yield per plant. Among all the characters, seed yield per plant and percentage of disease infection contributes maximum.</p> R. KARTHIKEYAN, R. THIRUMALAI, A.J. NAINU V. DIVYABHARATHI, R. NARAYANAN, S. MURUGAN ##submission.copyrightStatement## http://creativecommons.org/licenses/by/4.0 2018-11-15 2018-11-15 39 42 10.25081/hbr.2018.v4.3493