Studies on genetic variability of Capsicum frutescens var. Cakra Hijau induced by ethyl methane sulphonate (EMS) using SSR marker

Authors

  • Nur Rahmattullah Department of Biology, Faculty of Mathematics and Sciences, University of Brawijaya, Malang, Indonesia
  • Ramya Khrisnamurty Department of Biochemistry, Biotechnology and Bioinformatics, School of Biosciences, Avinashilingam Institute for Home Science and Higher Education for Women,Coimbatore- 641043, Tamil Nadu, India
  • Kalaiselvi Senthil Department of Biochemistry, Biotechnology and Bioinformatics, School of Biosciences, Avinashilingam Institute for Home Science and Higher Education for Women,Coimbatore- 641043, Tamil Nadu, India
  • Estri Laras Arumingtyas Department of Biology, Faculty of Mathematics and Sciences, University of Brawijaya, Malang, Indonesia

DOI:

https://doi.org/10.25081/cb.2020.v11.6303

Keywords:

Cayenne pepper, microsatellite loci, mutant pepper, wild type

Abstract

Genetic variability of wild type and EMS induced mutant cayenne peppers (Capsicum frutescens) var. Cakra Hijau is studied morphological traits and simple sequence repeat (SSR) markers. Phenotypic characters were investigated using standard pepper descriptors. The polymorphic SSR marker Ca19, Ca26, Ca52, Ca56, and Ca96were analyzed using unweighted pair group methods with arithmetic means (UPGMA) with Jaccard’s similarity index. The Polymorphic information content (PIC) value ranging from 0-0.228 with the highest index on Ca96. The Morphological traits showed of clusters (6 clades) with a lower cut-off value (0.2568) than SSR-based dendrogram did (4 clades; 0,5108). Therefore, genetic variability induced by EMS mutant were differentiated using morphological and SSR genotyping.

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Published

27-07-2020

How to Cite

Rahmattullah, N., Khrisnamurty, R., Senthil, K., & Arumingtyas, E. L. (2020). Studies on genetic variability of Capsicum frutescens var. Cakra Hijau induced by ethyl methane sulphonate (EMS) using SSR marker. Current Botany, 11, 125–131. https://doi.org/10.25081/cb.2020.v11.6303

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Section

Regular Articles