In vitro regeneration of two high-yielding eggplant (Solanum melongena L.) varieties of Bangladesh

  • Sabina Yesmin, Mst Muslima Khatun, Tanzena Tanny Plant Biotechnology Division, National Institute of Biotechnology, Ganakbari, Savar, Dhaka 1349, Bangladesh
  • Anica Tasnim Protity Microbial Biotechnology Division, National Institute of Biotechnology, Ganakbari, Savar, Dhaka 1349, Bangladesh
  • Md Salimullah Molecular Biotechnology Division, National Institute of Biotechnology, Ganakbari, Savar, Dhaka 1349, Bangladesh
  • Iftekhar Alam Plant Biotechnology Division, National Institute of Biotechnology, Ganakbari, Savar, Dhaka 1349, Bangladesh

Abstract

An in vitro regeneration protocol was developed for two high-yielding eggplant varieties (Solanum melongena L.) namely BARI begun-4 and BARI begun-6. Multiple shoots were regenerated from cotyledonary explants through organogenesis with growth regulators of different combinations and concentrations.  The best response towards multiple shoot regeneration was achieved from cotyledon explants on MS media complemented with 1 mg/l BAP + 0.2 mg/l IAA in both the two varieties of eggplant. Elongation of shoots was achieved on hormone free MS medium. Regenerated shoots of both the varieties produced   active in vitro root system on half strength of MS medium supplemented with 0.2 mg/l IBA.  The in vitro grown plantlets were acclimatized in soil, grew up to maturity, flowered, fruited and produced seeds as normal healthy plant like the control.

Downloads

Download data is not yet available.
Statistics
73 Views | 63 Downloads
How to Cite
Tanzena Tanny, S. Y. M. M. K., A. T. Protity, M. Salimullah, and I. Alam. “In Vitro Regeneration of Two High-Yielding Eggplant (Solanum Melongena L.) Varieties of Bangladesh”. Current Botany, Vol. 9,  , pp. 08-12, doi:https://doi.org/10.25081/cb.2018.v9.3376.
Section
Regular Articles