Antagonistic activity of coconut rhizospheric and endophytic Bacillus spp. against Ganoderma applanatum and Thielaviopsis paradoxa

Authors

  • Priya George Central Plantation Crops Research Institute Kudlu P.O., Kasaragod - 671 124
  • Alka Gupta Central Plantation Crops Research Institute Kudlu P.O., Kasaragod - 671 124
  • Murali Gopal Central Plantation Crops Research Institute Kudlu P.O., Kasaragod - 671 124
  • R. ChandraMohanan Central Plantation Crops Research Institute Kudlu P.O., Kasaragod - 671 124
  • Litty Thomas Central Plantation Crops Research Institute Kudlu P.O., Kasaragod - 671 124
  • George V. Thomas Central Plantation Crops Research Institute Kudlu P.O., Kasaragod - 671 124

Abstract

A screening study was carried out to detect the antagonistic potential of Bacillus spp. against Ganoderma applanatum and Thielaviopsis paradoxa, fungal pathogens of coconut. A total of 327 heat resistant, endospore producing bacilli were isolated from the rhizospheric soil and roots of coconut growing in Kerala, Tamil Nadu, Karnataka, Andhra Pradesh and Maharashtra. All the isolates were tested for antifungal activity against G. applanatum and T. paradoxa by dual cultural technique on nutrient agar medium. The zone of inhibition was measured and percentage of inhibition was calculated. More than 90 % of the rhizospheric and root endophytic isolates were found to effectively inhibit the mycelial growth of G. applanatum, with a maximum inhibition zone of 12 mm and percentage inhibition ranging from 44 to 91. About 86 % of the isolates inhibited the mycelial growth of T. paradoxa, with a maximum inhibition zone of 14 mm and percentage inhibition ranging from 42 to 93. Further tests of potent antagonists revealed that more than one mode of mechanisms like production of chitinase, siderophores, HCN, antibiotics, ammonia, β-1,3- glucanase and salicylic acid may be involved in the antagonistic activities. The results of this study revealed 13 Bacillus spp. having potential for use as biocontrol agents against G. applanatum and T. paradoxa, fungal pathogens of coconut.

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Published

27-06-2011

How to Cite

George, P., Gupta, A., Gopal, M., ChandraMohanan, R., Thomas, L., & Thomas, G. V. (2011). Antagonistic activity of coconut rhizospheric and endophytic Bacillus spp. against Ganoderma applanatum and Thielaviopsis paradoxa. Journal of Plantation Crops, 39(2), 278–284. Retrieved from https://updatepublishing.com/journal/index.php/JPC/article/view/5932

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Section

Research Articles